rat ccl19 beta antibody  (R&D Systems)

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: Time course of C-C chemokine ligand 19 (CCL19) mRNA expression in rats' TGs following the injection of nerve growth factor (NGF)-related reagents. (The statistical analysis results related to the image are shown in ).

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Expressing, Injection

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: p -value of C-C chemokine ligand 19 (CCL19) mRNA expression in rats' TGs following the injection of nerve growth factor (NGF)-related reagents.

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Expressing, Injection

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: ( A ) Immunofluorescence results of CCL19 protein expression after injection of NGF or normal saline into rats' trigeminal ganglia (TGs) 3 days after initiation of tooth movement. ( a ) Neurons expressing CCL19 (green) after injection of normal saline on day 3 after initiation of tooth movement. ( b ) Nuclei (blue) after injection of normal saline on day 3. ( c ) Overlap of ( A ) and ( B ). ( d–f ) The same labeling as in ( a–c ) but after injection of NGF on day 3. ( B ) Time course of CCL19 protein expression in TGs following the injection of NGF or normal saline after experimental tooth movement. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Immunofluorescence, Expressing, Injection, Saline, Labeling

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: ( A ) Calcitonin gene-related peptide (CGRP) protein expression levels in TGs following different interventions after experimental tooth movement. ( B ) CCL19 protein expression levels in TGs following different interventions after experimental tooth movement. ( C ) Comparison of immunofluorescence mean gray value of CGRP and CCL19 relative expression. ( D ) Immunofluorescence results of protein expression after injection on NGF, anti-NGF antibody, and normal saline into forced rats' TGs on the 3rd day after tooth movement. ( a, e, i ) Neurons expressing CGRP (green) after injection of related reagents. ( b, f, j ) Neurons expressing CCL19 (yellow). ( c, g, k ) Nuclei (blue) after injection of related reagents on day 3. ( d, h, l ) Overlap of ( a + b + c ), ( e + f + g ), and ( i + j + k ). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Expressing, Comparison, Immunofluorescence, Injection, Saline

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: Changes in pain expressed as the bite force of rats over time after injection of CCL19 and anti-CCL19 antibody. (The statistical analysis results related to the image are shown in ).

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Injection

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: p -value of bite force of rats over time after injection of CCL19 and anti-CCL19 antibody.

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Injection

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: Changes in pain expressed as the bite force modulated by NGF via CCL19 over time. (The statistical analysis results related to the image are shown in ).

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques:

Journal: Frontiers in Neurology

Article Title: Nerve Growth Factor Enhances Tooth Mechanical Hyperalgesia Through C-C Chemokine Ligand 19 in Rats

doi: 10.3389/fneur.2021.540660

Figure Lengend Snippet: p -value of bite force of rats over time after injection of CCL19/NGF-related reagents.

Article Snippet: This was followed by inoculations into the TGs of 10 μl of 0.6 μg/μl recombinant rat CCL19 beta protein (R&D Systems, USA) in PBS, 10 μl of 0.6 μg/μl rat CCL19 beta antibody (R&D Systems, USA) in PBS, or 10 μl of normal saline solution on days 1, 3, 5, 7, and 14.

Techniques: Injection